《植物生理学报》 2009, 45(6): 549-554

麻疯树中ADP 核糖基化因子基因的克隆和表达

林凡荣, 秦小波, 朱勋路, 叶生亮, 高继海, 徐莺, 陈放*

四川大学生命科学学院/ 生物资源与生态环境教育部重点实验室, 成都610064

通信作者：陈放；E-mail: scuchenfang@yahoo.com.cn；Tel: 028-85417281

摘　要：

从麻疯树 cDNA 文库中筛选到包含完整编码序列的 ADP 核糖基化因子的 cDNA 序列, 命名为 Jc-arf。其长度为 887 bp, 开放阅读框 546 bp, 推测的编码蛋白含 181 个氨基酸残基, 具有典型的 GTP 结合蛋白家族的特点: P 框(GLDAAGKT)、G’ 框(DVGGQ)和 G 框(NKQDL)。序列分析显示, Jc-arf 接近于人类 ClassI 型 arf 基因, 其蛋白序列与多种植物 ARF 有很高的同源性。半定量 RT-PCR 结果显示, Jc-arf 的表达具有一定的组织特异性, 在花中最丰富。PEG、低温、NaCl 胁迫下, Jc- arf 的表达受 PEG 和低温的诱导, 而对 NaCl 胁迫不敏感。

关键词：麻疯树; ADP 核糖基化因子; 表达; 胁迫

收稿：2009-01-09   修定：2009-05-20

资助：国家自然科学基金(30670204)、科技部国际合作项目(2006DFB63400)和“ 十一五” 科技支撑项目(2006BAD07A04)。

Cloning and Expression of an ADP-ribosylation Factor Gene in Jatropha curcas L.

LIN Fan-Rong, QIN Xiao-Bo, ZHU Xun-Lu, YE Sheng-Liang, GAO Ji-Hai, XU Ying, CHEN Fang*

Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China

Corresponding author: CHEN Fang; E-mail: scuchenfang@yahoo.com.cn; Tel: 028-85417281

Abstract:

An ADP-ribosylation factor gene, named Jc-arf, was cloned from Jatropha curcas. The cloned cDNA included a 546 bp complete open reading frame, which encoded a 181 amino acids peptid. This putative peptid contained three motifs: GLDAAGKT, DVGGQ and NKQDL, which involved in the feature of GTP-binding proteins. The alignment results showed Jc-ARF was high identity with many ARFs in other plants, and it was similar to ClassI of human ARFs. The semi-quantitative PCR results showed that Jc-arf expression was the highest in flowers. Further, the expression of Jc-arf could be induced in roots, stems and leaves by PEG, in roots under cool stress. However, it seemed insensitive to the NaCl stress.

Key words: Jatropha curcas; ADP-ribosylation factor; expression; stress